Goat anti-LIS1 / PAFAH1B1 Antibody

Pep ELISA, WB, IHC

Goat anti-LIS1 / PAFAH1B1 Antibody
Principle Name: LIS1 / PAFAH1B1
Official Symbol: PAFAH1B1
All Name and Symbols: PAFAH1B1; LIS1; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa; MDCR; PAFAH; lissencephaly 1 protein; Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit; platelet-activating factor acetylhydrolase, isoform I
Accession Number(s): NP_000421.1
Peptide Sequence: TGSVDQTVKVWECR, is from C Terminus
Human Gene ID(s): 5048
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 40kDa band observed in Human Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: Paraffin embedded Human Brain (Cortex), Thyroid and Pancreas. Recommended concentration: 3.75ug/ml.
Reference(s): Reiner O, Bar-Am I, Sapir T, Shmueli O, Carrozzo R, Lindsay EA, Baldini A, Ledbetter DH, Cahana A. LIS2, gene and pseudogene, homologous to LIS1 (lissencephaly 1), located on the short and long arms of chromosome 2. Genomics. 1995 Nov 20; 30(2):251-6.PMID: 8586424