Goat anti-APE1 / APEX1 Antibody

Pep ELISA, WB, IHC

Goat anti-APE1 / APEX1 Antibody
Principle Name: APE1 / APEX1
Official Symbol: APEX1
All Name and Symbols: APE1; APEX1; APEX; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; a
Accession Number(s): NP_001632.2
Peptide Sequence: PKRGKKGAVAEDGD, is from N Terminus
Human Gene ID(s): 328
Important Comments: Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6ug/ml.
Reference(s): Demple B, Herman T, Chen DS. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc Natl Acad Sci U S A. 1991 Dec 15; 88(24):11450-4.PMID: 1722334