Goat anti-KIF4A Antibody

Goat anti-KIF4A Antibody
Principle Name: KIF4A
Official Symbol: KIF4A
All Name and Symbols: KIF4A; kinesin family member 4A; FLJ12530; FLJ12655; FLJ14204; FLJ20631; HSA271784; KIF4; KIF4-G1; chromokinesin; kinesin family member 4
Accession Number(s): NP_036442.3
Peptide Sequence: SFFSGCSPIEEEAH, is from C Terminus
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, FC
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control:Reference(s): Sekine Y, Okada Y, Noda Y, Kondo S, Aizawa H, Takemura R, Hirokawa N. A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally. J Cell Biol. 1994 Oct; 127(1):187-201.PMID: 7929562