Goat anti-PRDM16 / MEL1 Antibody

Goat anti-PRDM16 / MEL1 Antibody
Principle Name: PRDM16 / MEL1
Official Symbol: PRDM16
All Name and Symbols: PRDM16; MEL1; KIAA1675; PR domain containing 16; transcription factor MEL1; MGC166915; PFM13; MDS1/EVI1-like; PR-domain zinc finger protein 16
Accession Number(s): NP_071397.2; NP_955533.1
Peptide Sequence: TSESGAFHPINHL, is from C Terminus
Human Gene ID(s): 63976
Important Comments: This antibody is expected to recognise both reported isoforms.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: No signal obtained yet but low background observed in Raji, Daudi, MOLT-4 and human brain lysates at up to 2ug/ml.
Reference(s): Mochizuki N, Shimizu S, Nagasawa T, Tanaka H, Taniwaki M, Yokota J, Morishita K. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1; 3)(p36; q21)-positive leukemia cells. Blood 2000 Nov 1; 96(9):3209-14.PMID: 11050005