Goat anti-MAD3 / MXD3 Antibody

Goat anti-MAD3 / MXD3 Antibody
Principle Name: MAD3 / MXD3
Official Symbol: MXD3
All Name and Symbols: MXD3; MAD3; MAX dimerization protein 3; MGC2383; hypothetical protein MGC2383; likely ortholog of mouse Max dimerization protein 3; BHLHC13; FLJ35523; MYX; Max-associated protein 3; Max-interacting transcriptional repressor MAD3
Accession Number(s): NP_112590.1
Peptide Sequence: QEHSYSHGGGAWL, is from C Terminus
Human Gene ID(s): 83463
Important Comments: This antibody is expected to recognise isoform a (NP_112590.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IF, IHC
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3ug/ml.
Immunohistochemistry: An anonymous customer found positive results in IHC on Human HeLa cells.
Reference(s): Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation. EMBO J. 1995 Nov 15; 14(22):5646-59.PMID: 8521822