Goat anti-SAE2 / UBA2 Antibody

Pep ELISA

Goat anti-SAE2 / UBA2 Antibody
Principle Name: SAE2 / UBA2
Official Symbol: SAE2
All Name and Symbols: UBA2; SAE2; SUMO-1 activating enzyme subunit 2
Accession Number(s): NP_005490
Peptide Sequence: ALSRGLPRELAEA, is from N Terminus
Human Gene ID(s): 10054
Non-Human GeneID(s): 50995 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: This antibody has been successfully used in a publication: PMID: 23832333. Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the
Reference(s): Desterro JM, Rodriguez MS, Kemp GD, Hay RT. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem. 1999 Apr 9; 274(15):10618-24.PMID: 10187858