Goat anti-FBXW2 Antibody

Pep ELISA, WB, IHC

Goat anti-FBXW2 Antibody
Principle Name: FBXW2
Official Symbol: FBXW2
All Name and Symbols: FBXW2; F-box and WD-40 domain protein 2; FBW2; Fwd2; MGC117371; Md6; F-box protein Fbw2; F-box and WD repeat domain containing 2
Accession Number(s): NP_036296.2
Peptide Sequence: KRGSSFLAGEHPG, is from C Terminus
Human Gene ID(s): 26190
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both band
Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous c
Reference(s): Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins. Curr Biol. 1999 Oct 21; 9(20):1180-2.PMID: 10531037