Goat anti-DSS1 / SHFM1 Antibody

Goat anti-DSS1 / SHFM1 Antibody
Principle Name: DSS1 / SHFM1
Official Symbol: SHFM1
All Name and Symbols: SHFM1; split hand/foot malformation (ectrodactyly) type 1; DSS1; Deleted in split-hand/split-foot 1 region; ECD; SEM1; SHFD1; SHSF1; Shfdg1; candidate for split hand/foot malformation type 1; deleted in split-hand/foot 1; deleted in split-hand/split-foot
Accession Number(s): NP_006295.1
Peptide Sequence: RAELEKHGYKMETS, is from C Terminus
Human Gene ID(s): 7979
Non-Human GeneID(s): 20422 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, ICC
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size
Reference(s): Wei SJ, Trempus CS, Cannon RE, Bortner CD, Tennant RW. Identification of Dss1 as a 12-O-Tetradecanoylphorbol-13-acetate-responsive Gene Expressed in Keratinocyte Progenitor Cells, with Possible Involvement in Early Skin Tumorigenesis. J Biol Chem. 2003 Jan 17; 278(3):1758-68.PMID: 12419822