Goat anti-INADL / PATJ Antibody

Goat anti-INADL / PATJ Antibody
Principle Name: INADL / PATJ
Official Symbol: INADL
All Name and Symbols: INADL; PATJ; PDZ domain protein (Drosophila inaD-like); protein associated to tight junctions; InaD-like (Drosophila); RP4-537K17.1; Cipp; FLJ26982; InaD-like; InaD-like; PALS1-associated tight junction protein; PDZ domain protein; channel-interacting PDZ
Accession Number(s): NP_795352.2
Peptide Sequence: PENPATDKLQVLQ, is from N Terminus
Human Gene ID(s): 10207
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments in Human Brain and Kidney lysates consistently showed a strong band at 100kDa followed by bands at ca.55kDa, 45kDa and 40kDa at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the
Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of spermatids. Recommended concentration, 3-6ug/ml.
Reference(s): Philipp S, Flockerzi V. Molecular characterization of a novel human PDZ domain protein with homology to INAD from Drosophila melanogaster. FEBS Lett. 1997 Aug 18; 413(2):243-8..PMID: 9280290