Goat anti-ARP1 homolog B Antibody

Pep ELISA, WB, IHC

Goat anti-ARP1 homolog B Antibody
Principle Name: ARP1 homolog B
Official Symbol: ACTR1B
All Name and Symbols: ACTR1B; ARP1B; CTRN2; ARP1 actin-related protein 1 homolog B, centractin beta (yeast); PC3; centractin beta; ARP1, yeast homolog B; ARP1 (actin-related protein 1, yeast) homolog B (centractin beta); ARP1 actin-related protein 1 homolog B, centractin beta
Accession Number(s): NP_005726.1
Peptide Sequence: YEEDGSRAIHRKT, is from C Terminus
Human Gene ID(s): 10120
Non-Human GeneID(s): 226977 (mouse);
Important Comments: This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2ug/ml.
Immunohistochemistry: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5ug/ml.
Reference(s): Clark SW, Staub O, Clark IB, Holzbaur EL, Paschal BM, Vallee RB, Meyer DI. Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. Mol Biol Cell. 1994 Dec; 5(12):1301-10.PMID: 7696711