Goat anti-ELF5 Antibody

Goat anti-ELF5 Antibody
Principle Name: ELF5
Official Symbol: ELF5
All Name and Symbols: ELF5; E74-like factor 5 (ets domain transcription factor); ESE2; ESE-2; E74-like factor 5; epithelium-specific Ets transcription factor 2
Accession Number(s): NP_938195.1; NP_001413.1, NP_001230009.1, NP_001230010.1
Peptide Sequence: GKNAHGWQEDKL, is from C Terminus
Human Gene ID(s): 2001
Non-Human GeneID(s): 13711 (mouse);
Important Comments: This antibody is expected to recognise reported Human isoforms represented by NP_938195.1, NP_001413.1, NP_001230009.1 and NP_001230010.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments in Human lysates of Lung, Kidney, Prostate and Testes gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Reference(s): Oettgen P, Kas K, Dube A, Gu X, Grall F, Thamrongsak U, Akbarali Y, Finger
E, Boltax J, Endress G, Munger K, Kunsch C, Libermann TA. Characterization of ESE-2, a novel ESE-1-related Ets transcription factor that is restricted to glandular epithelium and differentiated keratinocytes. J Biol Chem. 1999 Oct 8; 274(41):29439-52.PMID: 10506207