Goat anti-ADAM17 / TACE Antibody

Pep ELISA, WB, IHC

Goat anti-ADAM17 / TACE Antibody
Principle Name: ADAM17 / TACE
Official Symbol: ADAM17
All Name and Symbols: ADAM17; TACE; a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); CSVP; cSVP; CD156b; CD156b; MGC71942; TACE; cSVPADAM metallopeptidase domain 17; TNF-alpha converting enzyme; a disintegrin and metalloproteinas
Accession Number(s): NP_003174.3
Peptide Sequence: LQRQNRVDSKETEC, is from C Terminus
Human Gene ID(s): 6868
Non-Human GeneID(s): 11491 (mouse);
Important Comments: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in lite
Immunohistochemistry: Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75ug/ml.
Reference(s): Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature. 1997 Feb 20; 385(6618):729-33.PMID: 9034190