Goat anti-CDCP1 (isoform 1: C term) Antibody

Pep ELISA, WB, IHC

Goat anti-CDCP1 (isoform 1: C term) Antibody
Principle Name: CDCP1 (isoform 1: C term)
Official Symbol: CDCP1
All Name and Symbols: CDCP1; CUB domain containing protein 1 ; HGNC:24357; CD318; SIMA135; TRASK ; CUB domain-containing protein 1; transmembrane and associated with src kinases
Accession Number(s): NP_073753.3
Peptide Sequence: PLLNTQEPMEPAE, is from C Terminus
Human Gene ID(s): 64866
Important Comments: This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 200kDa. This band was succ
Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Reference(s): Hooper JD, Zijlstra A, Aimes RT, Liang H, Claassen GF, Tarin D, Testa JE, Quigley JP Subtractive immunization using highly metastatic human tumor cells identifies
SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003 Mar 27; 22(12):1783-94.PMID: 12660814