Goat anti-FHL2 Antibody

Pep ELISA, WB

Goat anti-FHL2 Antibody
Principle Name: FHL2
Official Symbol: FHL2
All Name and Symbols: FHL2; DRAL; AAG11; SLIM3; four and a half LIM domains 2; down-regulated in rhabdomyosarcoma LIM protein; aging-associated gene 11; four and a half LIM-domain protein 2; OTTHUMP00000203164; OTTHUMP00000203168; OTTHUMP00000203169
Accession Number(s): NP_001441.4
Peptide Sequence: RDDILCPDCGKDI, is from C Terminus
Human Gene ID(s): 2274
Non-Human GeneID(s): 14200 (mouse); 63839 (rat);
Important Comments: Reported variants represent identical protein (NP_001441.4; NP_963849.1; NP_963851.2; NP_001034581.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 30kDa band observed in Human and Mouse Heart lysates (calculated MW of 32.2kDa according to NP_001441.4). Recommended concentration: 1-3ug/ml.
Reference(s): Morgan MJ, Madgwick AJ. Slim defines a novel family of LIM-proteins expressed in skeletal muscle. Biochem Biophys Res Commun. 1996 Aug 14; 225(2):632-8.PMID: 8753811