Goat anti-TIF1A / TRIM24 Antibody

Pep ELISA, IHC

Goat anti-TIF1A / TRIM24 Antibody
Principle Name: TIF1A / TRIM24
Official Symbol: TRIM24
All Name and Symbols: TRIM24; TIF1; transcriptional intermediary factor 1; PTC6; TF1A; TIF1A; Tif1a; hTIF1; TIF1ALPHA; transcriptional intermediary factor 1 alpha; tripartite motif protein 24; tripartite motif-containing 24; RNF82
Accession Number(s): NP_056989.2; NP_003843.3
Peptide Sequence: KRLKSIEERQLLK, is from C Terminus
Human Gene ID(s): 8805
Important Comments: This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1ug/ml, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some o
Immunohistochemistry: In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6ug/ml.
Reference(s): Thenot S, Henriquet C, Rochefort H, Cavailles V. Differential interaction of nuclear receptors with the putative human transcriptional coactivator hTIF1. J Biol Chem. 1997 May 2; 272(18):12062-8.PMID: 9115274