Goat anti-PIK3C2A Antibody

Pep ELISA, IHC

Goat anti-PIK3C2A Antibody
Principle Name: PIK3C2A
Official Symbol: PIK3C2A
All Name and Symbols: PIK3C2A; CPK; PI3-K-C2A; PI3K-C2alpha; PI3-K-C2(ALPHA); phosphoinositide-3-kinase, class 2, alpha polypeptide; C2-containing phosphatidylinositol kinase; DKFZp686L193; MGC142218;
Accession Number(s): NP_002636.2
Peptide Sequence: TVKWYQLTAATYL, is from C Terminus
Human Gene ID(s): 5286
Non-Human GeneID(s): 18704 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1ug/ml.
Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Reference(s): Molz L, Chen YW, Hirano M, Williams LT. Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain. J Biol Chem. 1996 Jun 7; 271(23):13892-9.PMID: 8662856