Goat anti-Androgen Receptor Antibody

Goat anti-Androgen Receptor Antibody
Principle Name: Androgen Receptor
Official Symbol: AR
All Name and Symbols: AR; androgen receptor; dihydrotestosterone receptor; KD; AIS; TFM; DHTR; SBMA; NR3C4; SMAX1; HUMARA; HYSP1; androgen receptor; dihydrotestosterone receptor; RP11-383C12.1
Accession Number(s): NP_000035.2
Peptide Sequence: EVQLGLGRVYPRPPSC, is from N Terminus
Human Gene ID(s): 367
Non-Human GeneID(s): 11835 (mouse); 24208 (rat);
Important Comments: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, ChIP
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 95kDa band observed in Human Brain lysates (calculated MW of 99kDa according to NP_000035.2). Recommended concentration: 0.3-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this parti
Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml.
Reference(s): Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 1988 May 31; 153(1):241-8. .PMID: 3377788