Goat anti-CRKL Antibody

Pep ELISA, WB, IHC

Goat anti-CRKL Antibody
Principle Name: CRKL
Official Symbol: CRKL
All Name and Symbols: CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v-crk avian sarcoma virus CT10 oncogene homolog-like; OTTHUMP00000198539
Accession Number(s): NP_005198.1
Peptide Sequence: KIFDPQNPDENE, is from C Terminus
Human Gene ID(s): 1399
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa band observed in lysates of cell lines K562 (calculated MW of 33.8kDa according to NP_005198). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8ug/ml.
Reference(s): Singer CF, Hudelist G, Lamm W, Mueller R, Handl C, Kubista E, Czerwenka K. Active (p)CrkL is overexpressed in human malignancies: potential role as a
surrogate parameter for therapeutic tyrosine kinase inhibition. Oncol Rep. 2006 Feb; 15(2):353-9.PMID: 16391854