Goat anti-Prostaglandin dehydrogenase 1 Antibody

Pep ELISA, WB, IHC

Goat anti-Prostaglandin dehydrogenase 1 Antibody
Principle Name: Prostaglandin dehydrogenase 1
Official Symbol: HPGD
All Name and Symbols: HPGD; Prostaglandin dehydrogenase 1; PGDH1; 15-PGDH; hydroxyprostaglandin dehydrogenase 15-(NAD); PGDH; SDR36C1; NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 36C, member 1
Accession Number(s): NP_000851.2
Peptide Sequence: DYDTTPFQAKTQ, is from C Terminus
Human Gene ID(s): 3248
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated
Immunohistochemistry: In paraffin embedded Human Colon shows strong cytoplasm staining of enterocytes. Recommended concentration, 2-4ug/ml.
Reference(s): Coggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM, Koller BH. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med. 2002 Feb; 8(2):91-2. No abstract available. .PMID: 11821873