Goat anti-XAGE1 (Isoform 1d) / GAGED2 Antibody

Goat anti-XAGE1 (Isoform 1d) / GAGED2 Antibody
Principle Name: XAGE1 (Isoform 1d) / GAGED2
Official Symbol: GAGED2
All Name and Symbols: GAGED2; XAGE1; XAGE-1; G antigen, family D, 2; XAGE-1 protein
Accession Number(s): NP_597673.1
Peptide Sequence: CGFGFRRQGEDNT, is from C Terminus
Human Gene ID(s): 9503
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the produ
Immunohistochemistry: In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6ug/ml.
Reference(s): Liu XF, Helman LJ, Yeung C, Bera TK, Lee B, Pastan I. XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma. Cancer Res. 2000 Sep 1; 60(17):4752-5. .PMID: 10987281