Goat anti-FGFR1 Antibody

Pep ELISA, WB, IHC

Goat anti-FGFR1 Antibody
Principle Name: FGFR1
Official Symbol: FGFR1
All Name and Symbols: FGFR1; H2; H3; H4; H5; CEK; FLG; FLT2; KAL2; BFGFR; C-FGR; N-SAM; fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome); FLG protein; N-sam tyrosine kinase; tyrosylprotein kinase; protein-tyrosine kinase; FMS-like tyrosine
Accession Number(s): NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1
Peptide Sequence: CLPRHPAQLANGGLKR, is from C Terminus
Human Gene ID(s): 2260
Important Comments: This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 75kDa band observed in Human Breast lysates (calculated MW of 82.2kDa according to NP_075593, 81.9kDa according to NP_075594). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of the enterocytic cytoplasm. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 4ug/ml.
Reference(s): Torry DS, Mukherjea D, Arroyo J, Torry RJ. Expression and function of placenta growth factor: implications for abnormal placentation. J Soc Gynecol Investig. 2003 May; 10(4):178-88. Review. .PMID: 12759145