Goat anti-PAI1 / SERPINE1 Antibody

Goat anti-PAI1 / SERPINE1 Antibody
Principle Name: PAI1 / SERPINE1
Official Symbol: SERPINE1
All Name and Symbols: SERPINE1; PAI1; PAI; PAI-1; PLANH1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; plasminogen activator inhibitor, type I; serpin peptidase inhibitor, clade E (nexin, plasminogen activator in
Accession Number(s): NP_000593.1
Peptide Sequence: GFKIDDKGMAPALRH, is from internal region
Human Gene ID(s): 5054
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, EIA
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 48kDa band observed in Human Liver and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findin
Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6ug/ml.
A customer successfully used this product on Human Breast cancer.
Reference(s): Baumruker T, Csonga R, Pursch E, Pfeffer A, Urtz N, Sutton S, Bofill-Cardona E, Cooke M, Prieschl E. Activation of mast cells by incorporation of cholesterol into rafts. Int Immunol. 2003 Oct; 15(10):1207-18. .PMID: 13679390