Goat anti-SNAP25 Antibody

Pep ELISA, WB, IHC

Goat anti-SNAP25 Antibody
Principle Name: SNAP25
Official Symbol: SNAP25
All Name and Symbols: SNAP25; synaptosomal-associated protein, 25kDa ; HGNC:11132; FLJ23079; SNAP; SNAP-25; bA416N4.2; dJ1068F16.2 ; synaptosomal-associated protein 25; RIC-4; RIC4; SEC9; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein 25
Accession Number(s): NP_003072.2; NP_570824.1
Peptide Sequence: DEANQRATKMLGSG, is from C Terminus
Human Gene ID(s): 6616
Non-Human GeneID(s): 20614 (mouse); 25012 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 26kDa band observed in Human Brain and Mouse Brain lysates (calculated MW of 23.3kDa according to NP_003072.2 and NP_570824.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong axon staining, particularly in the granular region. Recommended concentration: 1-2ug/ml.
Reference(s): Voeller KK. Attention-deficit hyperactivity disorder (ADHD). J Child Neurol. 2004 Oct; 19(10):798-814. Review. .PMID: 15559895