Goat anti-Arrestin beta 2 Antibody

Pep ELISA, WB, IHC

Goat anti-Arrestin beta 2 Antibody
Principle Name: Arrestin beta 2
Official Symbol: ARRB2
All Name and Symbols: ARRB2; arrestin, beta 2; HGNC:712; ARB2; ARR2; DKFZp686L0365; arrestin beta 2; beta-arrestin 2; BARR2; arrestin 3
Accession Number(s): NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1
Peptide Sequence: HDHIPLPRPQS, is from internal region
Human Gene ID(s): 409
Non-Human GeneID(s): 216869 (mouse); 25388 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 40kDa band observed in Human Brain lysates (calculated MW of 46.1kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1ug/ml. A customer found positive results in WB on Rat aortic smooth mu
Immunohistochemistry: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration: 4-6ug/ml.
Reference(s): Lefkowitz RJ, Shenoy SK. Transduction of receptor signals by beta-arrestins. Science. 2005 Apr 22; 308(5721):512-7. Review. .PMID: 15845844