Goat anti-Ymer / CCDC50 Antibody

Pep ELISA, IHC

Goat anti-Ymer / CCDC50 Antibody
Principle Name: Ymer / CCDC50
Official Symbol: CCDC50
All Name and Symbols: CCDC50; Ymer protein; coiled-coil domain containing 50; C3orf6, YMER; C3orf6; chromosome 3 open reading frame 6; DFNA44
Accession Number(s): NP_848018.1; NP_777568.1
Peptide Sequence: CFSKSESSHKGFHYK, is from internal region
Human Gene ID(s): 152137
Important Comments: This antibody is expected to recognise both reported isoforms of human Ymer protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa
Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10ug/ml.
Reference(s): Blagoev B, Ong SE, Kratchmarova I, Mann M. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat Biotechnol. 2004 Sep; 22(9):1139-45. Epub 2004 Aug 15. .PMID: 15314609