Goat anti-APOE Antibody

Goat anti-APOE Antibody
Principle Name: APOE
Official Symbol: APOE
All Name and Symbols: APOE; AD2; MGC1571; apoprotein; apolipoprotein E; apolipoprotein E3; Alzheimer disease 2 (APOE*E4-associated, late onset); LPG
Accession Number(s): NP_000032.1
Peptide Sequence: VGTSAAPVPSDNH, is from C Terminus
Human Gene ID(s): 348
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx. 36kDa band observed in Human Brain lysates (calculated MW of 36.2kDa according to NP_000032.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Reference(s): Dodart JC, Marr RA, Koistinaho M, Gregersen BM, Malkani S, Verma IM, Paul SM. Gene delivery of human apolipoprotein E alters brain A{beta} burden in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A. 2005 Jan 25; 102(4):1211-6. Epub 2005 Jan 18. .PMID: 15657137