Goat anti-Makorin Antibody

Goat anti-Makorin Antibody
Principle Name: Makorin
Official Symbol: MKRN1
All Name and Symbols: MKRN1; makorin; ring finger protein 1; HGNC:7112; RNF61; makorin ring finger protein 1; FLJ21334
Accession Number(s): NP_038474.1; NP_001138597.1
Peptide Sequence: VTKEESEKEQTAVET, is from internal region
Human Gene ID(s): 23608
Non-Human GeneID(s):
Important Comments: This antibody may cross react with MKRN4 (52.9kDa).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag a
Reference(s): Kim JH, Park SM, Kang MR, Oh SY, Lee TH, Muller MT, Chung IK. Ubiquitin ligase MKRN1 modulates telomere length homeostasis through a proteolysis of hTERT. Genes Dev. 2005 Apr 1; 19(7):776-81. .PMID: 15805468