Goat anti-SPFH2 / ERLIN2 Antibody

Pep ELISA, WB, IHC

Goat anti-SPFH2 / ERLIN2 Antibody
Principle Name: SPFH2 / ERLIN2
Official Symbol: ERLIN2
All Name and Symbols: ERLIN2; SPFH2; ER lipid raft associated 2; SPFH domain family, member 2 ; HGNC:1356; C8orf2; MGC87072 ; chromosome 8 open reading frame 2; Erlin-2; MGC87072
Accession Number(s): NP_009106.1
Peptide Sequence: EDEPLETATKEN, is from C Terminus
Human Gene ID(s): 11160
Non-Human GeneID(s): 290823 (rat);
Important Comments: This antibody is expected to recognise isoform 1 (NP_009106) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast Cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibod
Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
Reference(s): Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C. A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes. Oncogene. 2005 Aug 4; 24(33):5235-45. .PMID: 15897872