Goat anti-SP1 Antibody

Pep ELISA, WB

Goat anti-SP1 Antibody
Principle Name: SP1
Official Symbol: SP1
All Name and Symbols: SP1; Sp1 transcription factor; HGNC:11205; specificity protein 1
Accession Number(s): NP_612482.2; NP_003100.1
Peptide Sequence: CSRIESPNENSNNSQ, is from internal region
Human Gene ID(s): 6667
Important Comments: This antibody is expected to recognise both reported isoforms (NP_612482.2; NP_003100.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 100kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 80.7kDa according to NP_612482.2). Recommended concentration: 0.3-1ug/ml.
Reference(s): Sakuntabhai A, Turbpaiboon C, Casademont I, Chuansumrit A, Lowhnoo T, Kajaste-Rudnitski A, Kalayanarooj SM, Tangnararatchakit K, Tangthawornchaikul N, Vasanawathana S, Chaiyaratana W, Yenchitsomanus PT, Suriyaphol P, Avirutnan P, Chokephaibulkit K, Matsud A variant in the CD209 promoter is associated with severity of dengue disease. Nat Genet. 2005 May; 37(5):507-13. Epub 2005 Apr 17. .PMID: 15838506