Goat anti-Cofilin 2 (muscle) Antibody

Goat anti-Cofilin 2 (muscle) Antibody
Principle Name: Cofilin 2 (muscle)
Official Symbol: CFL2
All Name and Symbols: cofilin 2 (muscle) ; HGNC:1875; CFL2; NEM7; cofilin 2
Accession Number(s): NP_068733.1; NP_619579.1
Peptide Sequence: DIKDRSTLGEK, is from C Terminus
Human Gene ID(s): 1073
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Rat
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 18kDa band observed in Human Skeletal Muscle lysates and in Rat Heart lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
Reference(s): Thirion C, Stucka R, Mendel B, Gruhler A, Jaksch M, Nowak KJ, Binz N, Laing NG, Lochmuller H. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem. 2001 Jun; 268(12):3473-82. .PMID: 11422377