Goat anti-Rhophilin 2 (Internal) Antibody

Pep ELISA, IHC

Goat anti-Rhophilin 2 (Internal) Antibody
Principle Name: Rhophilin 2 (Internal)
Official Symbol: RHPN2
All Name and Symbols: RHPN2; p76RBE; rhophilin, Rho GTPase binding protein 2; rhophilin 2; rhophilin-2; RhoB effector; rhophilin-like protein; P76RBE; RHOBP; 76 kDa RhoB effector protein; GTP-Rho-binding protein 2; rhophilin-like Rho-GTPase binding protein
Accession Number(s): NP_149094.3
Peptide Sequence: TNKNRQKSAST, is from internal region
Human Gene ID(s): 85415
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10ug/ml.
Reference(s): Peck JW, Oberst M, Bouker KB, Bowden E, Burbelo PD. The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization. J Biol Chem. 2002 Nov 15; 277(46):43924-32.PMID: 12221077