Goat anti-NANOG Antibody

Goat anti-NANOG Antibody
Principle Name: NANOG
Official Symbol: NANOG
All Name and Symbols: NANOG; Nanog homeobox; HGNC:20857 ; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
Accession Number(s): NP_079141.2
Peptide Sequence: QNQRMKSKRWQKNN, is from internal region
Human Gene ID(s): 79923
Non-Human GeneID(s): 71950 (mouse); 414065 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, IHC, EIA
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: This antibody was successfully used in Vassena et al, Hum Mol Genet. 2012 Aug 1; 21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22; 7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012; 21(5
Reference(s): Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Stem Cells. 2005 Sep; 23(8):1035-43. Epub 2005 Jun 27..PMID: 15983365