Goat anti-B5 Receptor / PCID1 / EIF3M Antibody

Pep ELISA, WB, IHC

Goat anti-B5 Receptor / PCID1 / EIF3M Antibody
Principle Name: B5 Receptor / PCID1 / EIF3M
Official Symbol: EIF3M
All Name and Symbols: EIF3M; B5 receptor; PCID1; PCI domain containing 1 (herpesvirus entry mediator); FLJ29030; GA17; hfl-B5; dendritic cell protein; fetal lung protein B5; eukaryotic translation initiation factor 3, subunit M; B5; PCID1
Accession Number(s): NP_006351.2
Peptide Sequence: NAWKQNLNKVKN, is from C Terminus
Human Gene ID(s): 10480
Non-Human GeneID(s): 98221 (mouse); 295975 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 38-40kDa band observed in lysates of human lung carcinoma cell line A549 (calculated MW of 42.5kDa according to NP_006351.2). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of cytoplasm in select spermatocytes. Recommended concentration, 5-10ug/ml.
Reference(s): Perez-Romero P, Fuller AO. The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection. J Virol. 2005 Jun; 79(12):7431-7. .PMID: 15919899