Goat anti-collagen type XI alpha 2 Antibody

Goat anti-collagen type XI alpha 2 Antibody
Principle Name: collagen type XI alpha 2
Official Symbol: COL11A2
All Name and Symbols: COL11A2; collagen, type XI, alpha 2; DAQB-79P13.8; HGNC:2187; DFNA13; HKE5; PARP; STL3; Collagen alpha 2(XI)
Accession Number(s): NP_542411.2; NP_542412.2; NP_542410.2; NP_001157243.1
Peptide Sequence: QKELECEGGQRERPQ, is from internal region
Human Gene ID(s): 1302
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_542411.2; NP_542412.2; NP_542410.2; NP_001157243.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Preliminary experiments in human brain and bone marrow lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported w
Reference(s): Harel T, Rabinowitz R, Hendler N, Galil A, Flusser H, Chemke J, Gradstein L, Lifshitz T, Ofir R, Elbedour K, Birk OS. COL11A2 mutation associated with autosomal recessive Weissenbacher-Zweymuller syndrome: molecular and clinical overlap with otospondylomegaepiphyseal dysplasia (OSMED). Am J Med Genet A. 2005 Jan 1; 132(1):33-5. .PMID: 15558753