Goat anti-Phosphodiesterase 4B Antibody

Pep ELISA, WB, IHC

Goat anti-Phosphodiesterase 4B Antibody
Principle Name: Phosphodiesterase 4B
Official Symbol: PDE4B
All Name and Symbols: PDE4B; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); RP5-876C12.1; DKFZp686F2182; DPDE4; MGC126529; PDEIVB ; OTTHUMP00000046896; cAMP-specific 3', 5'-cyclic phosphodiesterase 4B; dunce-like phosphodiesterase E4; phos
Accession Number(s): NP_002591.2; NP_001032418.1; NP_001032416.1; NP_001032417.1
Peptide Sequence: DIDIATEDKSPVDT, is from C Terminus
Human Gene ID(s): 5142
Important Comments: This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85kDa is obse
Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Reference(s): Millar JK, Pickard BS, Mackie S, James R, Christie S, Buchanan SR, Malloy MP, Chubb JE, Huston E, Baillie GS, Thomson PA, Hill EV, Brandon NJ, Rain JC, Camargo LM, Whiting PJ, Houslay MD, Blackwood DH, Muir WJ, Porteous DJ. DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling. Science. 2005 Nov 18; 310(5751):1187-91. .PMID: 16293762