Goat anti-GEF5 Antibody

Goat anti-GEF5 Antibody
Principle Name: GEF5
Official Symbol: ARHGEF5
All Name and Symbols: GEF5; DKFZp686N1969; ARHGEF5; Rho guanine nucleotide exchange factor (GEF) 5; P60; TIM; TIM1; guanine nucleotide regulatory protein TIM; oncogene TIM rho guanine nucleotide exchange factor 5; transforming immortalized mammary oncogene
Accession Number(s): NP_005426.2
Peptide Sequence: DKEIDQNSQQEE, is from internal region
Human Gene ID(s): 7984
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Reference(s): Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1; 13(3):323-34. Epub 2003 Dec 8. .PMID: 14662653