Goat anti-PCK1 / PEPCKC (internal) Antibody

Pep ELISA, IHC

Goat anti-PCK1 / PEPCKC (internal) Antibody
Principle Name: PCK1 / PEPCKC (internal)
Official Symbol: PCK1
All Name and Symbols: PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); HGNC:8724; MGC22652; PEPCK1; PEPCKC; PEP carboxykinase; cytosolic phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PEPCK-C
Accession Number(s): NP_002582.3
Peptide Sequence: HVNWFRKDKEGK, is from internal region
Human Gene ID(s): 5105
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration, 2-4ug/ml.
Reference(s): Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 2005 Jul 21; 436(7049):356-62. .PMID: 16034410