Goat anti-GLI3 Antibody

Goat anti-GLI3 Antibody
Principle Name: GLI3
Official Symbol: GLI3
All Name and Symbols: GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) ; ACLS; GCPS; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV ; DNA-binding protein; GLI-Kruppel family member GLI3; oncogene GLI3; zinc finger protein GLI3
Accession Number(s): NP_000159.2
Peptide Sequence: HNKRSKIKPDED, is from internal region
Human Gene ID(s): 2737
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments in Human Brain, Lung, Placenta and Testes lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been
Reference(s): Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005 Oct; 1(4):e53. Epub 2005 Oct 28. .PMID: 16254602