Goat anti-EIF2C1 / AGO1 Antibody

Goat anti-EIF2C1 / AGO1 Antibody
Principle Name: EIF2C1 / AGO1
Official Symbol: EIF2C1
All Name and Symbols: EIF2C1; eukaryotic translation initiation factor 2C, 1; AGO1; EIF2C; GERP95; Q99 ; Golgi Endoplasmic Reticulum protein 95 kDa; argonaute 1; putative RNA-binding protein Q99
Accession Number(s): NP_036331.1
Peptide Sequence: KNASYNLDPYIQEF, is from internal region
Human Gene ID(s): 26523
Non-Human GeneID(s):
Important Comments: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110kDa and 35kDa are observed. The 110kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2kDa according to NP_036331.1. Recom
Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 2.5ug/ml.
Reference(s): Gregory RI, Chendrimada TP, Cooch N, Shiekhattar R. Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell. 2005 Nov 18; 123(4):631-40. Epub 2005 Nov 3. .PMID: 16271387