Goat anti-PRODH Antibody

Pep ELISA, IHC

Goat anti-PRODH Antibody
Principle Name: PRODH
Official Symbol: PRODH
All Name and Symbols: PRODH; proline dehydrogenase (oxidase) 1 ; HSPOX2; PIG6; PRODH1; PRODH2; SCZD4; TP53I6; FLJ33744; MGC148078; MGC148079; Proline oxidase, mitochondrial; p53 induced protein; proline dehydrogenase (proline oxidase ); proline oxidase 2; tumor protein p53 ind
Accession Number(s): NP_057419.2
Peptide Sequence: ERDGSGTNKRDKQYQ, is from internal region
Human Gene ID(s): 5625
Non-Human GeneID(s): 19125 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave bands at approx 85kDa and 25kDa in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated siz
Immunohistochemistry: In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Recommended concentration, 3-6ug/ml.
Reference(s): Paterlini M, Zakharenko SS, Lai WS, Qin J, Zhang H, Mukai J, Westphal KG, Olivier B, Sulzer D, Pavlidis P, Siegelbaum SA, Karayiorgou M, Gogos JA. Transcriptional and behavioral interaction between 22q11.2 orthologs modulates schizophrenia-related phenotypes in mice. Nat Neurosci. 2005 Nov; 8(11):1586-94. Epub 2005 Oct 23. .PMID: 16234811