Goat anti-Hamartin / TSC1 (isoform 1) Antibody

Pep ELISA, WB, IHC

Goat anti-Hamartin / TSC1 (isoform 1) Antibody
Principle Name: Hamartin / TSC1 (isoform 1)
Official Symbol: TSC1
All Name and Symbols: TSC1; hamartin; tuberous sclerosis 1 ; HGNC:12362; KIAA0243; LAM; MGC86987; TSC; tuberous sclerosis 1 protein; tumor suppressor
Accession Number(s): NP_000359.1
Peptide Sequence: QLHIMDYNETHHEH, is from C Terminus
Human Gene ID(s): 7248
Non-Human GeneID(s): 64930 (mouse);
Important Comments: This antibody is expected to recognise isoform 1 (NP_000359.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000
Immunohistochemistry: In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration, 3-6ug/ml.
Reference(s): Chan JA, Zhang H, Roberts PS, Jozwiak S, Wieslawa G, Lewin-Kowalik J, Kotulska K, Kwiatkowski DJ. Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas: biallelic inactivation of TSC1 or TSC2 leads to mTOR activation. J Neuropathol Exp Neurol. 2004 Dec; 63(12):1236-42. .PMID: 15624760