Goat anti-CYP17A1 Antibody

Goat anti-CYP17A1 Antibody
Principle Name: CYP17A1
Official Symbol: CYP17A1
All Name and Symbols: CYP17A1; cytochrome P450, family 17, subfamily A, polypeptide 1 ; HGNC:2593; CPT7; CYP17; P450C17; S17AH; cytochrome P450, family 17; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome p450 XVIIA1; steroid 17-a
Accession Number(s): NP_000093.1
Peptide Sequence: LFKDGDQKLEK, is from internal region
Human Gene ID(s): 1586
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration: 3-5ug/ml.
Reference(s): Ohnishi T, Ogawa Y, Saibara T, Nishioka A, Kariya S, Fukumoto M, Onishi S, Yoshida S. CYP17 polymorphism as a risk factor of tamoxifen-induced hepatic steatosis in breast cancer patients. Oncol Rep. 2005 Mar; 13(3):485-9. .PMID: 15706422