Goat anti-MDM2 (isoform) Antibody

Goat anti-MDM2 (isoform) Antibody
Principle Name: MDM2 (isoform)
Official Symbol: MDM2
All Name and Symbols: MDM2; Mdm2; transformed 3T3 cell double minute 2; p53 binding protein (mouse); HGNC:6973; MGC71221; hdm2; mouse double minute 2 homolog; mouse double minute 2, human homolog of; p53-binding protein; p53-binding protein MDM2; ubiquitin-protein ligase E3 Md
Accession Number(s): NP_002383.2
Peptide Sequence: DELSGERQRKRHKSD, is from internal region
Human Gene ID(s): 4193
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48kDa, 60KDa, 70kDa and 90kDa are observed. These bands are not obser
Reference(s): Bond GL, Hu W, Bond EE, Robins H, Lutzker SG, Arva NC, Bargonetti J, Bartel F, Taubert H, Wuerl P, Onel K, Yip L, Hwang SJ, Strong LC, Lozano G, Levine AJ. A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans. Cell. 2004 Nov 24; 119(5):591-602. .PMID: 15550242