Goat anti-PGRMC1 / MPR Antibody

Goat anti-PGRMC1 / MPR Antibody
Principle Name: PGRMC1 / MPR
Official Symbol: PGRMC1
All Name and Symbols: PGRMC1; MPR; progesterone receptor membrane component 1 ; HGNC:16090; HPR6.6; progesterone binding protein
Accession Number(s): NP_006658.1
Peptide Sequence: EPKDESARKND, is from C Terminus
Human Gene ID(s): 10857
Non-Human GeneID(s): 53328 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 25kDa band observed in Human Kidney, Liver and Testis lysates (calculated MW of 21.7kDa according to NP_006658.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Engmann et al,
Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Reference(s): Hand RA, Craven RJ. Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells. J Cell Biochem. 2003 Oct 15; 90(3):534-47. .PMID: 14523988