Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Pep ELISA, WB, IHC

Goat anti-AIF1 / IBA1 (isoform 3) Antibody
Principle Name: AIF1 / IBA1 (isoform 3)
Official Symbol: AIF1
All Name and Symbols: AIF1; IBA1; allograft inflammatory factor 1 ; DASS-82G15.2; AIF-1; IRT-1; Em:AF129756.17; interferon gamma responsive transcript; ionized calcium-binding adapter molecule
Accession Number(s): NP_001614.3
Peptide Sequence: NKQFLDDPKYSSDED, is from internal region
Human Gene ID(s): 199
Non-Human GeneID(s): 11629 (mouse);
Important Comments: This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4ug/ml.
Reference(s): Yang ZF, Ho DW, Lau CK, Lam CT, Lum CT, Poon RT, Fan ST. Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages. Int Immunol. 2005 Nov; 17(11):1391-7. Epub 2005 Sep 12. .PMID: 16157606