Goat anti-HRS / HGS Antibody

Pep ELISA, IHC

Goat anti-HRS / HGS Antibody
Principle Name: HRS / HGS
Official Symbol: HGS
All Name and Symbols: HGS; hepatocyte growth factor-regulated tyrosine kinase substrate ; HGNC:4897; HRS; ZFYVE8; human growth factor-regulated tyrosine kinase substrate
Accession Number(s): NP_004703.1
Peptide Sequence: NRNYWEKKQEEARK, is from internal region
Human Gene ID(s): 9146
Non-Human GeneID(s): 15239 (mouse); 56084 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave bands at approx 140kDa and 26kDa in Human lysates of monocytic leukemia cell line U937 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands w
Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration: 1-2ug/ml.
Reference(s): Kobayashi H, Tanaka N, Asao H, Miura S, Kyuuma M, Semura K, Ishii N, Sugamura K. Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2. J Biol Chem. 2005 Mar 18; 280(11):10468-77. Epub 2005 Jan 7. .PMID: 15640163