Goat anti-GCDFP15 / PIP Antibody

Pep ELISA, WB, IHC

Goat anti-GCDFP15 / PIP Antibody
Principle Name: GCDFP15 / PIP
Official Symbol: PIP
All Name and Symbols: PIP; GCDFP15; prolactin-induced protein ; HGNC:8993; GCDFP-15; GP17; GPIP4; prolactin-inducible protein; gross cystic disease fluid protein 15
Accession Number(s): NP_002643.1
Peptide Sequence: GANKAQDNTRK, is from internal region
Human Gene ID(s): 5304
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration: 2-3ug/ml.
Reference(s): O'Connell FP, Wang HH, Odze RD. Utility of immunohistochemistry in distinguishing primary adenocarcinomas from metastatic breast carcinomas in the gastrointestinal tract. Arch Pathol Lab Med. 2005 Mar; 129(3):338-47. .PMID: 15737028