Goat anti-WNT3 Antibody

Goat anti-WNT3 Antibody
Principle Name: WNT3
Official Symbol: WNT3
All Name and Symbols: WNT3 ; wingless-type MMTV integration site family, member 3 ; INT4; MGC131950; MGC138321; MGC138323 ; WNT-3 proto-oncogene protein precursor
Accession Number(s): NP_110380.1
Peptide Sequence: CGRGHNTRTEKRKEK, is from internal region
Human Gene ID(s): 7473
Non-Human GeneID(s): 22415 (mouse); 24882 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4ug/ml.
Reference(s): Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y. Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping. Nature. 2006 Jan 5; 439(7072):31-7. .PMID: 16280981