Goat anti-Glutathione Peroxidase 1 (isoform1) Antibody

Pep ELISA, WB, IHC

Goat anti-Glutathione Peroxidase 1 (isoform1) Antibody
Principle Name: Glutathione Peroxidase 1 (isoform1)
Official Symbol: GPX1
All Name and Symbols: glutathione peroxidase 1 ; HGNC:4553; GPX1; GSHPX1; MGC14399; MGC88245
Accession Number(s): NP_000572.2
Peptide Sequence: REALPAPSDDATA, is from internal region
Human Gene ID(s): 2876
Important Comments: This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.
Frozen sections of Porcin Kidney shows staining of cyt
Reference(s): Kato S, Kato M, Abe Y, Matsumura T, Nishino T, Aoki M, Itoyama Y, Asayama K, Awaya A, Hirano A, Ohama E. Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models. Acta Neuropathol (Berl). 2005 Jun 28; [Epub ahead of print] .PMID: 15983830