Goat anti-CILP Antibody

Pep ELISA, WB

Goat anti-CILP Antibody
Principle Name: CILP
Official Symbol: CILP
All Name and Symbols: CILP; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase ; HGNC:1980; HsT18872 ; cartilage intermediate layer protein
Accession Number(s): NP_003604.2
Peptide Sequence: NYRRTDHEDPRVK, is from internal region
Human Gene ID(s): 8483
Non-Human GeneID(s): 214425 (mouse); 315761 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 150kDa band observed in rat spinal cord lysates (calculated MW of 133kDa according to human NP_003604.2 and of 132kDa according to Rat NP_001101631.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Seki S, Kawaguchi Y, Chiba K, Mikami Y, Kizawa H, Oya T, Mio F, Mori M, Miyamoto Y, Masuda I, Tsunoda T, Kamata M, Kubo T, Toyama Y, Kimura T, Nakamura Y, Ikegawa S. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat Genet. 2005 Jun; 37(6):607-12. Epub 2005 May 1. .PMID: 15864306